Common Bean (Phaseolus vulgaris) provides protein, complex carbohydrates, and other valuable nutrients for more than 300 million people in the tropics and around the world. Common bean is particularly known for its ability to provide fiber, with some studies showing that it aids in the lowering of blood sugar. It is also used as a model species for more complex plant species, as it is easy to grow and make genetic crosses for studying gene linkage. In this project, the goal was to identify if there was a significant difference in the quality and quantity of DNA isolated from fresh versus frozen leaf tissue. Fresh, young, trifoliate leaves were directly collected from newly grown common bean plants for DNA isolation using CTAB (Cetyltrimethylammonium bromide) buffer to help break down leaf tissue. The same procedure was used with common bean leaf tissue that had been stored in the -80 degrees deep freezer for more than five years. Differences in color, texture, and ease of grinding the leaf tissues were some physiological comparisons identified between fresh and frozen tissue. Both isolated DNA were run on a gel electrophoresis to visualize results. Of two fresh and frozen samples each, the results indicate that the frozen samples were more concentrated by visualization as well as quantification with a Nanodrop. Ultimately, the frozen tissue provided higher concentrations of DNA. Keywords: Common bean, DNA, CTAB, Gel Electrophoresis, Nanodrop Acknowledgements: We acknowledge USDA NIFA Capacity Building Grant Awards# 2016-06642 and 2016-06476 for funding my internship and the project as part of the student experiential learning goal and the Project Director, Dr. Gulnihal Ozbay for providing this internship opportunity.

Does Prolonged Storage of Plant Tissue have a Negative Effect on DNA Quality?

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Welcome to Delaware State University’s Annual Summer Research Symposium, and thank you for attending! Our symposium represents the culmination of students’ summer experiences in research at Delaware State University. This event showcases the work of college students working on faculty-led research projects, as well as middle and high school students experiencing research for the first time working in research groups. Whatever the case, this summer has been a unique experience for the students, and we hope a transformative one. Even for students who are not planning a career in scientific research, their summer experience has given them skills they will draw on for years to come. 
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DSU Summer Research Symposium 2021

Welcome to Delaware State University’s Annual Summer Research Symposium, and thank you for attending!

Dashawn Williams-Bey

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Does Prolonged Storage of Plant Tissue have a Negative Effect on DNA Quality?

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